Coding

Part:BBa_K4131003:Design

Designed by: Chun Ying Yu   Group: iGEM22_CCU_Taiwan   (2022-10-09)


Original pfa A gene (Moritella marina)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3993
    Illegal SpeI site found at 3508
    Illegal SpeI site found at 4966
    Illegal SpeI site found at 7075
    Illegal PstI site found at 1940
    Illegal PstI site found at 2882
    Illegal PstI site found at 4934
    Illegal PstI site found at 6425
    Illegal PstI site found at 7561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3993
    Illegal NheI site found at 3465
    Illegal NheI site found at 5260
    Illegal NheI site found at 6949
    Illegal SpeI site found at 3508
    Illegal SpeI site found at 4966
    Illegal SpeI site found at 7075
    Illegal PstI site found at 1940
    Illegal PstI site found at 2882
    Illegal PstI site found at 4934
    Illegal PstI site found at 6425
    Illegal PstI site found at 7561
    Illegal NotI site found at 5323
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3993
    Illegal BglII site found at 3948
    Illegal BglII site found at 4254
    Illegal BglII site found at 4860
    Illegal BglII site found at 6710
    Illegal BglII site found at 7370
    Illegal BamHI site found at 7648
    Illegal XhoI site found at 328
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3993
    Illegal SpeI site found at 3508
    Illegal SpeI site found at 4966
    Illegal SpeI site found at 7075
    Illegal PstI site found at 1940
    Illegal PstI site found at 2882
    Illegal PstI site found at 4934
    Illegal PstI site found at 6425
    Illegal PstI site found at 7561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3993
    Illegal SpeI site found at 3508
    Illegal SpeI site found at 4966
    Illegal SpeI site found at 7075
    Illegal PstI site found at 1940
    Illegal PstI site found at 2882
    Illegal PstI site found at 4934
    Illegal PstI site found at 6425
    Illegal PstI site found at 7561
    Illegal AgeI site found at 3814
    Illegal AgeI site found at 4120
    Illegal AgeI site found at 4726
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1993


Design Notes

To split the huge pfa A into two parts for cloning. We apply the endogenous EoCRI located at 3992 as the linking bridge.


Source

The sequence is extracted from the region 1632748-1640706 in Moritella marina genome (WP_019440098.1)

References